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KMID : 0903519960390040327
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1996 Volume.39 No. 4 p.327 ~ p.331
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Extraction Method of Anthocyanin and Tannin Pigments in Colored Rice
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김수언/Kim, Soo Un
오세관/최해춘/조미영/Oh, Sea Kwan/Choi, Hae Chune/Cho, Mi Yeong
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Abstract
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This experiment was conducted to establish the efficient extraction condition for anthocyanin and tannin pigments contained in rice bran of adored noes. Efficiency of the pigment extraction was maximum when the concentration of mixed solvent of methanol(3) : ethanul(7) was 70%. In purple rice(anthocyanin pigment), $quot;Kilimheugmi$quot;, 80% ethanol containing 0.5% magic acid showed the highest extraction efficiency and stability with a maximum absorbance wavelength(λ_(max)) at 538 ㎚. In red rice(tannin pigment). $quot;Jagwangdo$quot;. 80% ethanol containing 0.01% citric acid showed the highest extraction efficiency and stability with a maximum absorbance wavelength(λ_(max)) at 456 ㎚. The relative optical density of the pigments increased until the solvent temperature was reacted at 70℃, but drastically decreased over at 90℃ due to color change. The higher amount of the pigment was extracted from the longer shaking time of the solvent. Ten minutes was enough for the grinding time of rice bran in solvent. Supernatant of the pigment extractives after one day storage at 4℃ in Clark chamber revealed higher optical density than the filtration of the pigment extractives.
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